I also demonstrated that keratin structures of barbules are homogeneous in the central part of the eyespot, but have fibrous structures with many voids in the yellow-green parts. In contrast, melanin rods in the central part of the eyespot were only distributed in 1–3 layers on a part of the front side of the barbules. The melanin rods in the barbules reflecting yellow-green color comprise several ordered lattice structures.
The direction of the strokes really gives the illusion of feathers. Then I also added some white on his tail feathers, side feathers, back and neck. I added white on the top part of this head and blended it in with the rest of the pink. In addition, I compared microstructures of barbules in the central part of eyespot with those in surrounding areas using transmission electron microscope analysis. I think adding white made the flamingo really pop here. These analyses confirmed that barbules in the central part of the eyespot are in a horizontal position with respect to the barb, and that light transmission from the central part is less than that from the other parts. Herein, I examined various areas of eyespots on paraffin sections of feathers using an optical microscope, and characterized positional relationships between barbs and barbules. Under ultraviolet A (UVA), the central part of the eyespot reflects UVA better than the surrounding parts. Male peacock (Pavo cristatus) tail feathers have an eyespot pattern with an inconspicuous black or dark blue center surrounded by brilliant, structural colors, such as blue, light brown, and yellow-green.